Ultimate Peptide Phage Display

Ultimate Peptide Phage Display

Our libraries developed from precision DNA fragments have enabled us to generate a peptide phage display system with unmatched complexity. EPITOPIC‘s libraries have a complexity beyond one billion (109) variants, which would be only matched by a comparable standard peptide library with more than hundred fold more clones. The main advantage is the statistical even distribution of all amino acids present in the library. In combination with NGS (next generation sequencing) this allows skipping lengthy repeated selections and enables statistical analysis of data already from a single round of selection. By applying EPITOPIC’s proprietary sequence analysis on very large sets of selected phage display clones, we obtain comprehensive information about binding sequences. Using this information it is now possible to select binding motifs instead of complex testing of individual clones or peptides.
The advantage is beyond speed alone. EPITOPIC’s proprietary sequence analysis gives a much deeper insight into the specific characteristics of antigen recognition and reveals also the strength of individual antibodies when making a choice between different clones.
  • Reliable data without repeated selections
  • Reduced error effects from Next Generation Sequencing
  • In depth analysis based on hundreds or thousands of motif variants

FLAG-M1 motifFLAG-M2 motif

The power of EPITOPIC’s methods reveals already after a single selection round the essential differences between the monoclonal antibodies FLAG®-M1 (left) and the more specific FLAG®-M2 (right). These web-logos were generated on the basis of 1349 resp. 1935 different and 2644 resp. 4942 total sequences containing the DYK motif of the DYKDDDDK antigen used to generate these antibodies.

 

FLAG® and anti-FLAG® are registered trademarks of Sigma-Aldrich Co. LLC.

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