Publications

The following publications have applied the ENTE-1 library and epitopic’s software to generate epitope fingerprints:

1. Kunath J, Delaroque N, Szardenings M, Neundorf I, Straub RH. Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis. Life Sci. 2017 Jan 1;168:47-53. doi: 10.1016/j.lfs.2016.11.009. Epub 2016 Nov 14.

Although this publication is from the days we started evaluating NGS data with Excel data sheets, it still revealed interesting receptor epitopes.

2. Havenith H, Kern K, Rautenberger P, Spiegel H, Szardenings M, Ueberham E, Lehmann J, Buntru M, Vogel S, Treudler R, Fischer R, Schillberg S. Combination of two epitope identification techniques enables the rational design of soy allergen Gly m 4 mutants. Biotechnol J. 2017 Feb;12(2). doi: 10.1002/biot.201600441.

Comparing traditional peptide arrays and epitope fingerprinting with sera reveals insight into the structural function of an important food allergen.

3. Kern K, Havenith H, Delaroque N, Rautenberger P, Lehmann J, Fischer M, Spiegel H, Schillberg S, Ehrentreich-Foerster E, Aurich S, Treudler R, Szardenings M. The immunome of soybean allergy: Comprehensive identification and characterization of epitopes. Clin Exp Allergy. 2019 Feb;49(2):239-251. doi: 10.1111/cea.13285. Epub 2018 Dec 3. PMID: 30267550.

She did it all: Karolin Kern’s almost full mapping of the immune response in sensitized patients unravels a universe of epitopes with huge differences between individual persons and still only a few of them seem to be relevant.

4. Treudler R, Delaroque N, Puder M, Simon JC, Szardenings M. Dupilumab-induced Serum Sickness Like Reaction: An Unusual Adverse Effect in a patient with atopic eczema. J Eur Acad Dermatol Venereol. 2020 Jun 27. doi: 10.1111/jdv.16782. Online ahead of print. PMID: 32594596 DOI: 10.1111/jdv.16782

Acute Allergy: From serum sample to the actual epitope in less than three weeks! Thanks to faster evaluation tools the epitope mapping of the antibody and the epitopes involved in the allergic reaction could be identified quickly.

5. Ramírez Caballero L, Kny C, Treudler R, Simon JC, Kern K, Jappe U, Szardenings M. Identification of Seasonal Variations of Antibodies against PR-10-Specific Epitopes Can Be Improved Using Peptide-Phage Display. Int Arch Allergy Immunol. 2020 Aug 26:1-7. doi: 10.1159/000509995. Epub ahead of print. PMID: 32846424.

Even from sera: Sufficient data enable a statistical analysis of the seasonal changes in epitope preferences in differently sensitized patients.

Header – highlighted epitope sequence KANKD of anti-PTEN antibody (Perkin Elmer, Nr. AL380) in PTEN structure